The fragile histidine triad (FHIT) gene is a tumor suppressor gene that isaltered by deletion in a large fraction of human tumors, including pancreaticcancer. To evaluate the potential of FHIT gene therapy, we developed recombinant adenoviral and adenoassociated viral (AAV) FHIT vectors and tested these vectors in vitro and in vivo for activity against human pancreatic cancer cells. Our data show that viral FHIT gene delivery results in apoptosis by activation of thecaspase pathway. Furthermore, Fhit overexpression enhances the susceptibility of pancreatic cancer cells to exogenous inducers of apoptosis. In vivo results show that FHIT gene transfer delays tumor growth and prolongs survival in a murinemodel mimicking human disease.
Fragile histidine triad expression delays tumor development and induces apoptosis in human pancreatic cancer
TRAPASSO F;
2001-01-01
Abstract
The fragile histidine triad (FHIT) gene is a tumor suppressor gene that isaltered by deletion in a large fraction of human tumors, including pancreaticcancer. To evaluate the potential of FHIT gene therapy, we developed recombinant adenoviral and adenoassociated viral (AAV) FHIT vectors and tested these vectors in vitro and in vivo for activity against human pancreatic cancer cells. Our data show that viral FHIT gene delivery results in apoptosis by activation of thecaspase pathway. Furthermore, Fhit overexpression enhances the susceptibility of pancreatic cancer cells to exogenous inducers of apoptosis. In vivo results show that FHIT gene transfer delays tumor growth and prolongs survival in a murinemodel mimicking human disease.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
