Rose petal-derived nanovesicles as a sustainable and functional ingredient for next-generation cosmetics

Nanovesicles derived from natural sources are gaining increasing attention as innovative and sustainable ingredients for advanced cosmetic formulations. In this study, we explored rose petal-derived nanovesicles (RPNVs) as a novel topical delivery system with potential anti-aging benefits. RP-NVs were isolated through mechanical disruption of Rosa spp. petals followed by a standardized purification process combining differential centrifugation and size exclusion chromatography. The resulting vesicles exhibited a size distribution ranging between 50 and 300 nm and a net negative surface charge, making them suitable for cutaneous application. Physicochemical characterization confirmed their colloidal stability, while in vitro tests on human keratinocytes demonstrated excellent cytocompatibility and significant antioxidant activity. RP-NVs were successfully incorporated into a face cream which was optimized to preserve proper rheological features and physical stability, as confirmed by Turbiscan analysis and dynamic rheological investigation, carried out at room temperature and 40 degrees C. In vivo evaluation on human volunteers showed suitable skin tolerance and suggested that the formulations maintained skin homeostasis, as highlighted by stable values of erythema index, transepidermal water loss (TEWL) and hydration values after application. These data also aligned with subjective skin-feeling evaluations, confirming perceived skin comfort and compatibility. Overall, RP-NVs may represent a promising plantderived ingredient for advanced cosmetic formulations designed to support skin barrier function, mitigate oxidative stress, and contribute to skin wellness and aging prevention.