The degree to which liposomes are PEGylated is the feature, which most influences the length of the presence of stealth liposomes in the bloodstream. In order to thoroughly investigate the maximum amount of DSPE-PEG(2000) that can be used to stabilize stealth liposomes, these were synthesized at different concentrations of DSPE-PEG(2000) and their physicochemical properties were investigated by using differential scanning calorimetry (DSC). The kinetics of PEGylation and de-PEGylation were performed by incubating non-stealth liposomes in a DSPE-PEG(2000) suspension at different incubation times, and then analyzing the data using DSC and dynamic light scattering (DLS) techniques. The results demonstrated that DSPE-PEC2000 was self-assembled in the phospholipid bilayers, thus forming stealth liposomes. The different amounts of DSPE-PEG(2000) in the bilayer triggered a de-PEGylation phenomenon, resulting in mixed nanoaggregates, which derived from the detergent-like properties of the PEGylated phospholipids. (C) 2017 Elsevier B.V. All rights reserved.
Interaction between PEGylated Lipid and Phospholipids: an Insight of PEGylation Degree and Kinetics of de-PEGylation
Cristiano M. C.;Cosco D;Fresta M;Paolino D
2017-01-01
Abstract
The degree to which liposomes are PEGylated is the feature, which most influences the length of the presence of stealth liposomes in the bloodstream. In order to thoroughly investigate the maximum amount of DSPE-PEG(2000) that can be used to stabilize stealth liposomes, these were synthesized at different concentrations of DSPE-PEG(2000) and their physicochemical properties were investigated by using differential scanning calorimetry (DSC). The kinetics of PEGylation and de-PEGylation were performed by incubating non-stealth liposomes in a DSPE-PEG(2000) suspension at different incubation times, and then analyzing the data using DSC and dynamic light scattering (DLS) techniques. The results demonstrated that DSPE-PEC2000 was self-assembled in the phospholipid bilayers, thus forming stealth liposomes. The different amounts of DSPE-PEG(2000) in the bilayer triggered a de-PEGylation phenomenon, resulting in mixed nanoaggregates, which derived from the detergent-like properties of the PEGylated phospholipids. (C) 2017 Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
