Estrogen receptors alpha (ER-alpha) and beta (ER-beta) play distinct biological roles in onset and progression of hormone-responsive breast cancer, with ER-beta exerting a modulatory activity on ER-alpha-mediated estrogen signaling and stimulation of cell proliferation by mechanisms still not fully understood. We stably expressed human ER-beta fused to a tandem affinity purification-tag in estrogen-responsive MCF-7 cells and applied tandem affinity purification and nanoLC-MS/MS to identify the ER-beta interactome of this cell type. Functional annotation by bioinformatics analyses of the 303 proteins that co-purify with ER-beta from nuclear extracts identify several new molecular partners of this receptor subtype that represents nodal points of a large protein network controlling multiple processes and functions in breast cancer cells.

A large set of estrogen receptor beta-interacting proteins identified by tandem affinity purification in hormone-responsive human breast cancer cell nuclei

Guzzi PH;Cannataro M;
2011-01-01

Abstract

Estrogen receptors alpha (ER-alpha) and beta (ER-beta) play distinct biological roles in onset and progression of hormone-responsive breast cancer, with ER-beta exerting a modulatory activity on ER-alpha-mediated estrogen signaling and stimulation of cell proliferation by mechanisms still not fully understood. We stably expressed human ER-beta fused to a tandem affinity purification-tag in estrogen-responsive MCF-7 cells and applied tandem affinity purification and nanoLC-MS/MS to identify the ER-beta interactome of this cell type. Functional annotation by bioinformatics analyses of the 303 proteins that co-purify with ER-beta from nuclear extracts identify several new molecular partners of this receptor subtype that represents nodal points of a large protein network controlling multiple processes and functions in breast cancer cells.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12317/12704
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