Nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS) has become an essential tool in the field of proteomics. In fact, its sensitivity has advantages over conventional LC-MS/MS that allow the analysis of peptide mixtures in sample-limited situations (e.g., proteolytically digested proteins isolated by two-dimensional gel electrophoresis). Technical challenges, associated with low flow rates of the chromatographic separation, make this technology still difficult to run routinely. Here, we describe a nano LC-MS/MS setup that allows several weeks of continuous operation for the analysis of peptides derived by enzymatic digestion of either purified proteins or moderately complex protein mixtures.
Nano LC–MS/MS: A Robust Setup for Proteomic Analysis
Gaspari M;Cuda G
2011-01-01
Abstract
Nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS) has become an essential tool in the field of proteomics. In fact, its sensitivity has advantages over conventional LC-MS/MS that allow the analysis of peptide mixtures in sample-limited situations (e.g., proteolytically digested proteins isolated by two-dimensional gel electrophoresis). Technical challenges, associated with low flow rates of the chromatographic separation, make this technology still difficult to run routinely. Here, we describe a nano LC-MS/MS setup that allows several weeks of continuous operation for the analysis of peptides derived by enzymatic digestion of either purified proteins or moderately complex protein mixtures.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
