Background. Adult stem cell-derived cardiomyocyte cells may be a promising source of cells for applications in regenerative medicine, including cardiovascular tissue engineering and primary progenitor cells from native vessels may have limited proliferative capacity and reduced collagen production. Methods. Bone-marrow blood samples were collected by patients in need of vascular grafts due to coronary disease or peripheral arterial disease. Based upon our findings regarding growth factors and cytokines a two-phases culture protocol including a 2 week proliferation and 2 week differentiation phase was develop to optimize proliferation and cardiomyocytes differentiation of human mesenchymal stem cells (MSCs) cons cutely. Results. Our work showed the ability of human bone-marrow-derived mesenchymal stem cells to differentiate by various growth factors and cytokines. Efficient recovery of MSCs from cryopreserved intact teeth and second-passage MSCs cultures was achieved. These studies indicate that MSCs isolation is feasible for patients submitted to cardiovascular surgery, and imply that processing immediately after blood collection is required for successful banking of MScs. Further, the recovery of viable MScs after cryopreservation of intact teeth suggests that minimal processing may be needed for the banking of samples with no immediate plans for expansion and use. Conclusions. These initial studies will facilitate the development of future protocols for the clinical banking of MSC

USE OF HUMAN MESENCHYMAL STEM CELLS, BONE-MARROW BLOODDERIVED, AS AN ALTERNATIVE SOURCE OF CARDIOMYOCYTE CELLS FOR TISSUE ENGINEERING

N. Malara;V. Mollace;G. F. Serraino;Mastroroberto P
2014-01-01

Abstract

Background. Adult stem cell-derived cardiomyocyte cells may be a promising source of cells for applications in regenerative medicine, including cardiovascular tissue engineering and primary progenitor cells from native vessels may have limited proliferative capacity and reduced collagen production. Methods. Bone-marrow blood samples were collected by patients in need of vascular grafts due to coronary disease or peripheral arterial disease. Based upon our findings regarding growth factors and cytokines a two-phases culture protocol including a 2 week proliferation and 2 week differentiation phase was develop to optimize proliferation and cardiomyocytes differentiation of human mesenchymal stem cells (MSCs) cons cutely. Results. Our work showed the ability of human bone-marrow-derived mesenchymal stem cells to differentiate by various growth factors and cytokines. Efficient recovery of MSCs from cryopreserved intact teeth and second-passage MSCs cultures was achieved. These studies indicate that MSCs isolation is feasible for patients submitted to cardiovascular surgery, and imply that processing immediately after blood collection is required for successful banking of MScs. Further, the recovery of viable MScs after cryopreservation of intact teeth suggests that minimal processing may be needed for the banking of samples with no immediate plans for expansion and use. Conclusions. These initial studies will facilitate the development of future protocols for the clinical banking of MSC
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12317/21097
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