Method for detection of HMGAI (high mobility group AI) gene polymorphism, consisting of an heterozygous sin­ gle-nucleotide C insertion at position -13 of exon 6 (C.l36-l4_136-l3insC), wherein: a) a DNA sample of the proband subject is extracted; b) it is amplified by PCR (Polymerase Chain Reaction) using specific primers; c) it is then sequenced by means of the same primers and a diagnostic method of determining whether an individual has an increased risk of developing insulin resistance and/or type 2 diabetes mellitus comprising the steps of: a) obtaining a biological sample comprising a nucleic acid (DNA) from said individual; b) amplifYing a region of said HMGAl (high mobility group Al) in said DNA comprising said polymorphic site, and c) detecting the presence or absence of the HMGAI nucleotide sequence variant in said sample to obtain a variant profile of said individual, wherein said nucleotide sequence variant is at position -13 of exon 6 (C.l36-l4_136-l3insC).

METHOD FOR DETECTION AND DIAGNOSTIC METHOD OF HMGAI GENE POLYMORPHISM

BRUNETTI A;DANIELA FOTI;
2010-01-01

Abstract

Method for detection of HMGAI (high mobility group AI) gene polymorphism, consisting of an heterozygous sin­ gle-nucleotide C insertion at position -13 of exon 6 (C.l36-l4_136-l3insC), wherein: a) a DNA sample of the proband subject is extracted; b) it is amplified by PCR (Polymerase Chain Reaction) using specific primers; c) it is then sequenced by means of the same primers and a diagnostic method of determining whether an individual has an increased risk of developing insulin resistance and/or type 2 diabetes mellitus comprising the steps of: a) obtaining a biological sample comprising a nucleic acid (DNA) from said individual; b) amplifYing a region of said HMGAl (high mobility group Al) in said DNA comprising said polymorphic site, and c) detecting the presence or absence of the HMGAI nucleotide sequence variant in said sample to obtain a variant profile of said individual, wherein said nucleotide sequence variant is at position -13 of exon 6 (C.l36-l4_136-l3insC).
2010
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12317/22263
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