The anions (I-III) obtained from O-methyl 5'-O-(5'-deoxythymidine) 3'- O-(2',3'-dideoxyuridine) phosphate by the competitive removal of the 3-N-H protons of the nucleobases and of the methyl group from the phosphotriester bond, assume in the gas phase stable conformations as a function of their charge site. The mass-analyzed ion kinetic energy (MIKE) spectra of I and III show that the regioselective backbone cleavage of the internucleotidic linkage is controlled by the 2'-H proton transfer to the nucleobase within the 5'-end nucleoside. Similar pathways are taken by species II when the nucleobase is eliminated as neutral from the 5'-end nucleoside.

Participation of the nucleobases in the regioselective backbone fragmentation of nucleic acids. A molecular dynamics and tandem mass spectrometric investigation on a model dinucleoside phosphotriester

De Nino A.;Procopio A.;Sindona G.
1997-01-01

Abstract

The anions (I-III) obtained from O-methyl 5'-O-(5'-deoxythymidine) 3'- O-(2',3'-dideoxyuridine) phosphate by the competitive removal of the 3-N-H protons of the nucleobases and of the methyl group from the phosphotriester bond, assume in the gas phase stable conformations as a function of their charge site. The mass-analyzed ion kinetic energy (MIKE) spectra of I and III show that the regioselective backbone cleavage of the internucleotidic linkage is controlled by the 2'-H proton transfer to the nucleobase within the 5'-end nucleoside. Similar pathways are taken by species II when the nucleobase is eliminated as neutral from the 5'-end nucleoside.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12317/59231
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 6
  • ???jsp.display-item.citation.isi??? ND
social impact