EXPOSURE of adult rabbits to darkness for 48 h produces bilateral DNA fragmentation in the lateral geniculate nucleus as revealed in brain sections by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labelling (TUNEL) technique, suggesting an apoptotic type of cell death. In agreement with the latter deduction, light microscopy assessment of the morphological characteristics showed marginalization and condensation of nuclear chromatin, typical features of apoptosis. These effects were abolished by systemic administration of Nω-nitro-L-arginine methyl ester, an inhibitor of nitric oxide (NO) synthesis, whereas the D isomer was ineffective. In conclusion, the present data demonstrate that light deprivation for 48 h produces apoptosis in the lateral geniculate nucleus of rabbit and suggest that NO might be involved.

Nω-nitro-L-arginine methyl ester prevents apoptosis induced in the lateral geniculate nucleus by light deprivation in adult rabbit

Palma E.;
1996-01-01

Abstract

EXPOSURE of adult rabbits to darkness for 48 h produces bilateral DNA fragmentation in the lateral geniculate nucleus as revealed in brain sections by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labelling (TUNEL) technique, suggesting an apoptotic type of cell death. In agreement with the latter deduction, light microscopy assessment of the morphological characteristics showed marginalization and condensation of nuclear chromatin, typical features of apoptosis. These effects were abolished by systemic administration of Nω-nitro-L-arginine methyl ester, an inhibitor of nitric oxide (NO) synthesis, whereas the D isomer was ineffective. In conclusion, the present data demonstrate that light deprivation for 48 h produces apoptosis in the lateral geniculate nucleus of rabbit and suggest that NO might be involved.
1996
Apoptosis
DNA fragmentation
Lateral geniculate nucleus (LGN)
N
ω
-nitro-l-arginine methyl ester (L-NAME)
Rabbit
Animals
Apoptosis
DNA Fragmentation
Enzyme Inhibitors
Geniculate Bodies
Histocytochemistry
Male
NG-Nitroarginine Methyl Ester
Nitric Oxide Synthase
Rabbits
Darkness
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.12317/63844
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