The Leaf Volatile Constituents of Isatis tinctoria by Solid-Phase Microextraction and Gas Chromatography/Mass Spectrometry

The leaf volatile constituents of Jsatis tinctoria L (Brassicaceae) have been studied by Solid-Phase. Microextraction and Gas chromatography/Mass Spectrometry (SPME/GC-MS). Seventy components were fully characterized by mass spectra, linear retention indices, and injection of standards; the average composition (ppm) as single components and classes of substances is reported. Aliphatic hydrocarbons, acids, alcohols, aldehydes and esters, aromatic aldehydes, esters and ethers, furans, isothiocyanates and thiocyanates, sulfurated compounds, nitriles, terpenes and sesquiterpenes were identified. Leaf volatiles in Isatis tinctoria L were characterized by a high amount of isothiocyanates which accounted for about 40% of the total volatile fraction. Isothiocya¬nates are important and characteristic flavour compounds in Brassica vegetables and the cancer chemo-protective attributes are recently responsible for their growing interest